Evaluating CMR

Imaging Techniques

Computed Tomography (CT)

Determining Tissue Area or Mass Using CT


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CT images are normally analyzed using one of two methods: 1) the perimeter of the tissue is traced manually (3) and the area within the perimeter is calculated by multiplying the number of pixels in the region of interest by their known area, or 2) image segmentation algorithms are used to identify all pixels within a selected range of intensities (i.e., HU) believed to be representative of a specific tissue (4). The latter method is more common when assessing CT images. 

Because of the radiation involved with CT, multiple CT images are generally not acquired. However, if multiple images are acquired, tissue volumes are calculated with the same methods used for MRI images (5-8). They can then be converted to mass units by multiplying the volume by the assumed density values for that tissue (9, 10).  


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3. Abate N, Burns D, Peshock RM, et al. Estimation of adipose tissue mass by magnetic resonance imaging: validation against dissection in human cadavers. J Lipid Res 1994; 35: 1490-6.
4. Mourier A, Gautier JF, De Kerviler E, et al. Mobilization of visceral adipose tissue related to the improvement in insulin sensitivity in response to physical training in NIDDM. Effects of branched-chain amino acid supplements. Diabetes Care 1997; 20: 385-91.
5. Kvist H, Sjostrom L and Tylen U. Adipose tissue volume determinations in women by computed tomography: technical considerations. Int J Obes 1986; 10: 53-67.
6. Ross R, Rissanen J, Pedwell H, et al. Influence of diet and exercise on skeletal muscle and visceral adipose tissue in men. J Appl Physiol 1996; 81: 2445-55.
7. Ross R. Magnetic resonance imaging provides new insights into the characterization of adipose and lean tissue distribution. Can J Physiol Pharmacol 1996; 74: 778-85.
8. Shen W, Wang Z, Tang H, et al. Volume estimates by imaging methods: model comparisons with visible women as the reference. Obes Res 2003; 11: 217-25.
9. Snyder WS, Cooke MJ, Manssett ES, et al. Report of the Task Group on Reference Man. 1975.
10. Gallagher D, Belmonte D, Deurenberg P, et al. Organ-tissue mass measurement allows modeling of REE and metabolically active tissue mass. Am J Physiol 1998; 275: E249-58.

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