CT images are normally analyzed using one of two methods: 1) the perimeter of the tissue is traced manually (3) and the area within the perimeter is calculated by multiplying the number of pixels in the region of interest by their known area, or 2) image segmentation algorithms are used to identify all pixels within a selected range of intensities (i.e., HU) believed to be representative of a specific tissue (4). The latter method is more common when assessing CT images.
Because of the radiation involved with CT, multiple CT images are generally not acquired. However, if multiple images are acquired, tissue volumes are calculated with the same methods used for MRI images (5-8). They can then be converted to mass units by multiplying the volume by the assumed density values for that tissue (9, 10).